Practical 5 : Propagation of seaweed in enrichment media

Introduction

Seaweeds are large and microscopic form. It is also marine macroalgae and has simple reproductive structures. Besides, seaweed do not have tissues for conduction (non- vescular). There are three basic parts of seaweed which are balde, stipe and holdfast. Then, there is various type of seaweed that played important role in environment system. The importance of seaweed is as bioremediator, food sources to human and animal, and biomolecules. Each group was given Glacilaria changii as sample for this experiment and different media each group.  

Sample

Glacilaria changii

Media

Sterile seawater, Von Stosch’s Enrichment (VSE) , Provasoli Enrichment Seawater (PES) media.

Bacteria culture

17 difference culture   *3 replicates

Steps

1. Sample washed with tap water carefully.
2. Sample was cut 1 cm by 1 cm

   

   Figure 1: cut sample 1 cm

3. Then, weight sample one by one

   

   Figure 2: weight sample with electronic weighing 

4.  wash sample with sterile seawater ( for 30 second)

   

   Figure 3: Sample was washed.

5. Well –plate was filled with sterile seawater
6. 10⁴ CFU/ ml of selected bacteria was added (our group used bacteria 13,14,15, and 16

   

   Figure 4: Selected bacteria was added in each spaces. 

7.  Next, seaweed was put in well- plate

   

   Figure 5: seaweed was put in well- plate.
8. The sample was observed every seven days

Result  

               

              Day 1st 

                 Day 7th 

B1 sample buds already grow on it.

Buds grown

          Day 14th 

Almost all of the seaweed grow bud on it

One of the sample that buds grown on it

Another samples